5GS6
Full-length NS1 structure of Zika virus from 2015 Brazil strain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97853 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 96.948, 96.948, 270.143 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.474 - 2.852 |
R-factor | 0.2457 |
Rwork | 0.244 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o6b |
RMSD bond length | 0.004 |
RMSD bond angle | 0.937 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.850 |
Number of reflections | 30984 |
<I/σ(I)> | 24.152 |
Completeness [%] | 100.0 |
Redundancy | 14.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1M Tris hydrochloride pH 8.5, 2.0M ammonium sulfate |