5GS5
Crystal structure of apo rat STING
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-17 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.1000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.290, 75.510, 87.640 |
| Unit cell angles | 90.00, 115.35, 90.00 |
Refinement procedure
| Resolution | 42.728 - 1.840 |
| R-factor | 0.1833 |
| Rwork | 0.181 |
| R-free | 0.22190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4loj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.292 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.760 | |
| Number of reflections | 70041 | |
| <I/σ(I)> | 12.54 | 2.23 |
| Completeness [%] | 98.8 | 98.2 |
| Redundancy | 3.75 | 3.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 200mM NaCl, 20mM MES pH6.5 |
