5GRV

Crystal structure of homo-specific diabody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	80
Detector technology	CCD
Collection date	2013-04-11
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 61 2 2
Unit cell lengths	134.124, 134.124, 76.269
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.232 - 2.300
R-factor	0.1853
Rwork	0.183
R-free	0.22090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gs1
RMSD bond length	0.009
RMSD bond angle	1.370
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	18614
<I/σ(I)>	24.2
Completeness [%]	99.9
Redundancy	17.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	296	0.1 M MES pH 6.5, 2.3 M Sodium acetate, 5% glycerol