5GRA

Crystal structure of TrmJ from Z. mobilis ZM4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97952
Spacegroup name	C 1 2 1
Unit cell lengths	174.232, 41.811, 72.633
Unit cell angles	90.00, 91.46, 90.00

Refinement procedure

Resolution	40.656 - 3.005
R-factor	0.2341
Rwork	0.232
R-free	0.26900
RMSD bond length	0.003
RMSD bond angle	0.816
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000
Number of reflections	19496
<I/σ(I)>	4.1
Completeness [%]	97.7
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	Ammonium Sulfate, MES, Lithium Sulfate