5GR8
Crystal structure of PEPR1-AtPEP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-30 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.490, 96.967, 106.013 |
| Unit cell angles | 90.00, 110.79, 90.00 |
Refinement procedure
| Resolution | 49.553 - 2.587 |
| R-factor | 0.2372 |
| Rwork | 0.234 |
| R-free | 0.28840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mn8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.616 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.630 |
| High resolution limit [Å] | 2.587 | 2.590 |
| Number of reflections | 58543 | |
| <I/σ(I)> | 43 | |
| Completeness [%] | 89.7 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.2 M Trimethylamine N-oxide dehydrate, 0.1 M Tris, 20% w/v Polyethylene Glycol Monomethyl ether 2000 |
