5GR7

Mouse MHC class I H-2Kd with a MERS-CoV-derived peptide 37-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-07-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	50.006, 74.644, 121.129
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.322 - 2.400
R-factor	0.2037
Rwork	0.201
R-free	0.25680
RMSD bond length	0.003
RMSD bond angle	0.696
Data scaling software	CNS
Phasing software	PHASES
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	168813
<I/σ(I)>	24.673
Completeness [%]	97.4
Redundancy	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.1M Sodium citrate tribasic dihydrate pH 5.5, 16% w/v Polyethylene glycol 8000