5GQX
Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-27 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 134.025, 134.025, 185.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.380 - 2.300 |
R-factor | 0.15076 |
Rwork | 0.149 |
R-free | 0.18422 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gqw |
RMSD bond length | 0.021 |
RMSD bond angle | 2.194 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.075 | 0.261 |
Number of reflections | 75270 | |
<I/σ(I)> | 52.5 | 15.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.5 | 14.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | magnesium chloride, ethanol, HEPES-NaOH |