5GQU
Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 133.750, 133.750, 185.902 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.290 - 1.850 |
R-factor | 0.14802 |
Rwork | 0.147 |
R-free | 0.17024 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m7x |
RMSD bond length | 0.028 |
RMSD bond angle | 2.340 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.074 | 0.274 |
Number of reflections | 143511 | |
<I/σ(I)> | 61 | 10.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.6 | 14.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | magnesium chloride, ethanol, HEPES-NaOH |