5GQO
Structure of the second Single Stranded DNA Binding protein (SSBb) from Mycobacterium smegmatis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-08-16 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5419 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 73.610, 73.610, 216.210 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.240 - 2.500 |
R-factor | 0.21334 |
Rwork | 0.211 |
R-free | 0.25627 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eiv |
RMSD bond length | 0.016 |
RMSD bond angle | 1.775 |
Data reduction software | iMOSFLM (7.0.9) |
Data scaling software | SCALA |
Phasing software | PHASER (2.5.5) |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.240 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.093 | 0.100 |
Number of reflections | 12839 | |
<I/σ(I)> | 16.4 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.9 | 9.6 |
CC(1/2) | 0.999 | 0.671 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 298 | 0.2M calcium chloride dihydrate, 0.1M HEPES pH 7.5, 7.5 % (v/v) PEG 400, 3% (w/v) 1,5-diaminopentane dihydrochloride |