5GQN

Crystal structure of in cellulo Cypovirus Polyhedra mutant with deletion of Gly192-Ala194

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-25
Detector	RAYONIX MX225HE
Wavelength(s)	1
Spacegroup name	I 2 3
Unit cell lengths	103.320, 103.320, 103.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.180 - 1.550
R-factor	0.15222
Rwork	0.148
R-free	0.18720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oh6
RMSD bond length	0.022
RMSD bond angle	2.023
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.650
High resolution limit [Å]	1.550	1.550
Number of reflections	26733
<I/σ(I)>	7.63
Completeness [%]	100.0
Redundancy	21.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	IN CELL		300	in vivo crystallization