5GQ0

Crystal structure of the Epithiospecifier Protein, ESP from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	100.127, 100.127, 164.814
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.004 - 2.310
R-factor	0.1906
Rwork	0.189
R-free	0.23050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a10
RMSD bond length	0.008
RMSD bond angle	1.177
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.370
High resolution limit [Å]	2.308	2.310
Rmerge	0.139
Number of reflections	37659
<I/σ(I)>	22.5
Completeness [%]	99.4
Redundancy	21.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2M potassium sodium tartrate tetrahydrate pH 7.4, 20% PEG3350