5GPY

Crystal structure of the human TFIIE complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-02
Detector	RAYONIX MX-225
Wavelength(s)	1.282446
Spacegroup name	P 21 21 21
Unit cell lengths	51.520, 69.200, 116.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.325 - 2.100
R-factor	0.2007
Rwork	0.199
R-free	0.23720
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.006
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	SHELXD
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.500	2.210
High resolution limit [Å]	2.100	2.100
Rmerge		0.752
Number of reflections	24970
<I/σ(I)>	6.3	1.4
Completeness [%]	99.9	100
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.9	293	12% PEG3350