5GPY
Crystal structure of the human TFIIE complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-02 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.282446 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.520, 69.200, 116.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.325 - 2.100 |
| R-factor | 0.2007 |
| Rwork | 0.199 |
| R-free | 0.23720 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.006 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.500 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.752 | |
| Number of reflections | 24970 | |
| <I/σ(I)> | 6.3 | 1.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.9 | 293 | 12% PEG3350 |
