5GPL
Crystal structure of Ccp1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MACSCIENCE |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-01 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97892 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 86.662, 86.662, 158.771 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.756 - 2.100 |
| R-factor | 0.1952 |
| Rwork | 0.193 |
| R-free | 0.23560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gpk |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.731 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 36027 | |
| <I/σ(I)> | 23.8 | |
| Completeness [%] | 99.6 | |
| Redundancy | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 289 | 100mM sodiam manolate pH4.0 200mM NH4NO3 18% PEG 3,350 15% EG |
