5GOP
Crystal structure of alkaline invertase InvA from Anabaena sp. PCC 7120 complexed with sucrose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97916 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 99.866, 178.923, 181.324 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.941 - 2.350 |
| R-factor | 0.208 |
| Rwork | 0.207 |
| R-free | 0.23530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5goo |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.084 |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.340 |
| Number of reflections | 67492 | |
| <I/σ(I)> | 17.2 | 2.9 |
| Completeness [%] | 98.5 | 99.7 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.5 M Lithium sulfate, 0.1 M Tris |
