5GOI
Lys48-linked di-ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-E |
| Synchrotron site | APS |
| Beamline | 21-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.010, 40.800, 52.160 |
| Unit cell angles | 98.45, 100.90, 105.94 |
Refinement procedure
| Resolution | 38.351 - 1.594 |
| R-factor | 0.2665 |
| Rwork | 0.264 |
| R-free | 0.31060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.783 |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.351 | 1.640 |
| High resolution limit [Å] | 1.594 | 1.590 |
| Rmerge | 0.089 | 1.086 |
| Number of reflections | 23441 | |
| <I/σ(I)> | 4 | |
| Completeness [%] | 82.0 | |
| Redundancy | 1.69 | 1.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1M Sodium citrate tribasic dehydrate PH5.6, 20% 2-Propanol, 20%PEG 4000 |
