5GOH
Lys33-linked di-ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-17 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.436, 29.200, 44.232 |
| Unit cell angles | 83.14, 86.82, 71.18 |
Refinement procedure
| Resolution | 27.479 - 1.946 |
| R-factor | 0.2376 |
| Rwork | 0.236 |
| R-free | 0.27120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.095 |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.479 | 2.020 |
| High resolution limit [Å] | 1.946 | 1.950 |
| Rmerge | 0.076 | 0.291 |
| Number of reflections | 8490 | |
| <I/σ(I)> | 16 | |
| Completeness [%] | 93.0 | |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M Magnesium sulfate heptahydrate, 20% peg 3350 |
