5GOG

Lys29-linked di-ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-E
Synchrotron site	APS
Beamline	21-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 1 2 1
Unit cell lengths	28.027, 46.497, 43.157
Unit cell angles	90.00, 97.74, 90.00

Refinement procedure

Resolution	46.497 - 1.977
R-factor	0.2495
Rwork	0.245
R-free	0.32970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq
RMSD bond length	0.019
RMSD bond angle	1.847
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.497	2.050
High resolution limit [Å]	1.977	1.980
Rmerge	0.117	0.382
Number of reflections	5293
<I/σ(I)>	7.32
Completeness [%]	68.0
Redundancy	2.5	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M Potassium sulfate, 20% PEG 3350