5GOE

Truncated mitofusin-1, GDP-bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-12
Detector	RAYONIX MX-225
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	70.420, 72.910, 94.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.693 - 1.801
R-factor	0.1784
Rwork	0.177
R-free	0.20420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.763
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	44.700
High resolution limit [Å]	1.800
Number of reflections	86503
<I/σ(I)>	25.08
Completeness [%]	98.9
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M ammonium citrate tribasic and 20% PEG 3350