5GOD

Lys27-linked di-ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-E
Synchrotron site	APS
Beamline	21-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1
Unit cell lengths	38.240, 38.260, 48.000
Unit cell angles	98.15, 98.15, 109.67

Refinement procedure

Resolution	35.275 - 1.150
R-factor	0.2031
Rwork	0.202
R-free	0.22150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq
RMSD bond length	0.006
RMSD bond angle	0.980
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.275	1.190
High resolution limit [Å]	1.150	1.150
Rmerge	0.034	0.604
Number of reflections	67808
<I/σ(I)>	11.42
Completeness [%]	77.0
Redundancy	1.93	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M Magnesium acetate tetrahydrate, 0.1M Sodium cacodylate trihydrate PH6.5, 20% PEG 8000