5GOC
Lys11-linked diubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-07 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.523, 26.733, 43.768 |
| Unit cell angles | 75.17, 76.00, 80.96 |
Refinement procedure
| Resolution | 25.709 - 1.733 |
| R-factor | 0.1891 |
| Rwork | 0.187 |
| R-free | 0.23550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.084 |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.710 | 1.800 |
| High resolution limit [Å] | 1.733 | 1.730 |
| Rmerge | 0.060 | 0.176 |
| Number of reflections | 7987 | |
| <I/σ(I)> | 8.43 | |
| Completeness [%] | 69.0 | |
| Redundancy | 1.8 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M Li2SO4, 0.1M tris 8.5, 30% PEG 4000 |
