5GOB
Lys6-linked di-ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-E |
| Synchrotron site | APS |
| Beamline | 21-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 24.730, 26.380, 45.120 |
| Unit cell angles | 98.07, 93.85, 106.54 |
Refinement procedure
| Resolution | 44.388 - 1.153 |
| R-factor | 0.1931 |
| Rwork | 0.191 |
| R-free | 0.22850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.014 |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.390 | 1.200 |
| High resolution limit [Å] | 1.153 | 1.160 |
| Rmerge | 0.066 | 0.749 |
| Number of reflections | 23485 | |
| <I/σ(I)> | 11.25 | |
| Completeness [%] | 62.0 | |
| Redundancy | 3.54 | 3.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 289 | 0.2M Magnesium chloride hexahydrate, 20% PEG 3350, PH5.9 |
