5GNX
The E171Q mutant structure of Bgl6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-07-13 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 125.247, 95.697, 94.976 |
Unit cell angles | 90.00, 125.61, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.12419 |
Rwork | 0.123 |
R-free | 0.15117 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oin |
RMSD bond length | 0.016 |
RMSD bond angle | 1.442 |
Data reduction software | PHENIX |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.042 | 0.081 |
Number of reflections | 84462 | |
<I/σ(I)> | 48.3 | 25.8 |
Completeness [%] | 98.5 | 99.8 |
Redundancy | 3.7 | 3.7 |
CC(1/2) | 0.990 | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 25% PEG 8K, 600 mM NaCl, 100 mM Tris-HCl pH 8.0, 1M glucose |