5GNV
Structure of PSD-95/MAP1A complex reveals unique target recognition mode of MAGUK GK domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97928 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 62.445, 156.861, 59.513 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.089 - 2.596 |
R-factor | 0.2171 |
Rwork | 0.215 |
R-free | 0.25710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.491 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 9354 |
<I/σ(I)> | 31.8 |
Completeness [%] | 99.8 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 289 | 0.2M lithium sulfate, 0.1M Bis-Tris pH 6.5 and 25%(w/v) polyethylene glycol 3350 |