5GNU
the structure of mini-MFN1 apo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.001, 76.490, 95.356 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.198 - 4.113 |
R-factor | 0.2907 |
Rwork | 0.284 |
R-free | 0.34990 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.561 |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 4.100 |
Number of reflections | 4483 |
<I/σ(I)> | 13.5 |
Completeness [%] | 99.0 |
Redundancy | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 200mM ammonium sulfate, 100mM Tris (pH 8.5), and 25%(w/v) PEG 3350 |