5GNU

the structure of mini-MFN1 apo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	73.001, 76.490, 95.356
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.198 - 4.113
R-factor	0.2907
Rwork	0.284
R-free	0.34990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.561
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	4.100
Number of reflections	4483
<I/σ(I)>	13.5
Completeness [%]	99.0
Redundancy	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	200mM ammonium sulfate, 100mM Tris (pH 8.5), and 25%(w/v) PEG 3350