5GNT

BDLP-like folding of Mitofusin 1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	70.361, 72.527, 94.071
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.495 - 2.665
R-factor	0.2093
Rwork	0.206
R-free	0.27380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.486
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.660
Number of reflections	14293
<I/σ(I)>	56.7
Completeness [%]	99.9
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	200 mM sodium sulfate decahydrate and 20% (w/v) PEG 3350