5GNR
the structure of mini-MFN1 K88A in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9785 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.340, 74.975, 94.451 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.959 - 2.650 |
R-factor | 0.2248 |
Rwork | 0.216 |
R-free | 0.30160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.522 |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.650 |
Number of reflections | 15269 |
<I/σ(I)> | 21.38 |
Completeness [%] | 99.8 |
Redundancy | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 200 mM ammonium fluoride, 20% (w/v) PEG 3350 |