5GNJ
Structure of a transcription factor and DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 77.100, 79.700, 102.800 |
Unit cell angles | 90.00, 105.00, 90.00 |
Refinement procedure
Resolution | 49.649 - 2.700 |
R-factor | 0.2091 |
Rwork | 0.206 |
R-free | 0.27330 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.435 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.650 | 2.700 | |
High resolution limit [Å] | 2.660 | 30.000 | 2.660 |
Rmerge | 0.053 | 0.031 | 0.563 |
Number of reflections | 32765 | ||
<I/σ(I)> | 13.02 | 15.73 | 1.51 |
Completeness [%] | 94.1 | 15.6 | 61.5 |
Redundancy | 2.4 | ||
CC(1/2) | 0.998 | 0.629 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.2 M Calcium chloride dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 20% v/v 2-Propanol |