5GNF
Crystal structure of anti-CRISPR protein AcrF3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 65 |
Unit cell lengths | 84.944, 84.944, 63.155 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.472 - 1.500 |
R-factor | 0.1711 |
Rwork | 0.170 |
R-free | 0.19190 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.781 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.065 | 0.403 |
Number of reflections | 41528 | |
<I/σ(I)> | 43.8 | 3.4 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 15.4 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 289 | 20% (w/v) PEG 8000, 100mM MES, 200mM Calcium acetate |