5GN3

Structure of selenomethionine-labelled Uracil DNA glycosylase (BdiUNG) from Bradyrhizobium diazoefficiens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	93
Detector technology	CCD
Collection date	2016-07-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	70.956, 90.068, 256.269
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.990 - 2.281
R-factor	0.1808
Rwork	0.180
R-free	0.21200
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.193
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.990	2.320
High resolution limit [Å]	2.281	2.281
Number of reflections	132492
<I/σ(I)>	21.39
Completeness [%]	91.7
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	20% PEG3350, 200 mM sodium citrate, 100 mM sodium citrate/citric acid