5GN2

Crystal structure of Uracil DNA glycosylase (BdiUNG) from Bradyrhizobium diazoefficiens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	93
Detector technology	CCD
Collection date	2016-07-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	70.652, 90.031, 255.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.524 - 1.952
R-factor	0.2462
Rwork	0.245
R-free	0.28980
RMSD bond length	0.009
RMSD bond angle	1.256
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.524
High resolution limit [Å]	1.952
Number of reflections	117654
<I/σ(I)>	13.34
Completeness [%]	98.7
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	20% PEG3350, 200 mM sodium citrate, 100 mM sodium citrate/citric acid