5GN1

Crystal structure of the C-terminal part of Fun30 ATPase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-20
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.1000
Spacegroup name	C 1 2 1
Unit cell lengths	114.431, 118.394, 111.578
Unit cell angles	90.00, 107.28, 90.00

Refinement procedure

Resolution	36.205 - 1.950
R-factor	0.1877
Rwork	0.187
R-free	0.21630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mwy
RMSD bond length	0.010
RMSD bond angle	1.021
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.950	1.950
Number of reflections	103556
<I/σ(I)>	20.73	2.13
Completeness [%]	100.0	100
Redundancy	6.7	6.8
CC(1/2)		0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	289	0.1 M Sodium malonate, 12% PEG 3350