5GMY
Crystal structure of the Archaeoglobus fulgidus oligosaccharyltransferase (O29867_ARCFU) tethered with an acceptor peptide containing the NVT sequon via a disulfide bond
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-10 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 121.549, 121.549, 181.344 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.100 - 3.500 |
R-factor | 0.2282 |
Rwork | 0.226 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wak |
RMSD bond length | 0.002 |
RMSD bond angle | 0.561 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.900 | 3.560 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.106 | 1.283 |
Number of reflections | 17649 | 828 |
<I/σ(I)> | 44.7 | 3.6 |
Completeness [%] | 99.8 | 100 |
Redundancy | 13.9 | 14.2 |
CC(1/2) | 0.956 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10 mM MgCl2, 0.1 M Bis-Tris, pH 6.5, 22 % (w/v) PEG 550MME, 5% (v/v) Jeffamine M-600 |