5GML

Crystal Structure of GRASP Domain of GRASP55 with N terminal extra residues

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	I 41 2 2
Unit cell lengths	132.312, 132.312, 221.055
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	93.559 - 2.546
R-factor	0.264
Rwork	0.261
R-free	0.31060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rle
RMSD bond length	0.008
RMSD bond angle	1.261
Data reduction software	HKL-2000
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.560	2.637
High resolution limit [Å]	2.546	2.546
Rmerge	0.131	0.980
Number of reflections	32268
<I/σ(I)>	14.53	3.57
Completeness [%]	99.4	99.34
Redundancy	13.4	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	sodium formate, sodium acetate, etc