5GMB

Methylation at position 32 of tRNA catalyzed by TrmJ alters oxidative stress response in Pseudomonas aeruiginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	110
Detector technology	CCD
Collection date	2015-03-26
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	59.796, 59.796, 124.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.580 - 1.620
R-factor	0.179
Rwork	0.178
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cnd
RMSD bond length	0.010
RMSD bond angle	1.020
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.030	1.650
High resolution limit [Å]	1.620	1.620
Rmerge	0.060	0.730
Number of reflections	29132
<I/σ(I)>	19	3.9
Completeness [%]	95.4	71.5
Redundancy	9.9	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293.15	MgCl2, CaCl2, PEG MME 550, PEG 20000, MOPS/Na-HEPES pH7.5