5GLW
Tl-gal with LacNAc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-06-24 |
Detector | MAC Science DIP-320 |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.823, 84.193, 78.413 |
Unit cell angles | 90.00, 109.51, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
Rwork | 0.238 |
R-free | 0.27680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hl0 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.053 | 0.146 |
Number of reflections | 50857 | |
<I/σ(I)> | 30.9 | |
Completeness [%] | 94.0 | |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% polyethylene glycol 8000, 100mM Na/K phosphate (pH 6.2), 200mM NaCl |