5GLN
Crystal structure of CoXyl43, GH43 beta-xylosidase/alpha-arabinofuranosidase from a compostmicrobial metagenome in complex with xylotriose, calcium-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2015-05-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.760, 61.587, 78.922 |
| Unit cell angles | 90.00, 95.84, 90.00 |
Refinement procedure
| Resolution | 78.510 - 1.700 |
| R-factor | 0.15858 |
| Rwork | 0.158 |
| R-free | 0.17352 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gll |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.267 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.095 | 0.605 |
| Number of reflections | 78466 | |
| <I/σ(I)> | 21.5 | 3.4 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 7.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 25% PEG 3350, 8% Tacsimate |
