5GLL
Crystal structure of CoXyl43, GH43 beta-xylosidase/alpha-arabinofuranosidase from a compostmicrobial metagenome, calcium-bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2015-05-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.490, 60.935, 78.901 |
Unit cell angles | 90.00, 95.05, 90.00 |
Refinement procedure
Resolution | 78.590 - 1.800 |
R-factor | 0.15906 |
Rwork | 0.158 |
R-free | 0.18520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mlg |
RMSD bond length | 0.007 |
RMSD bond angle | 1.264 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.140 | 0.838 |
Number of reflections | 64759 | |
<I/σ(I)> | 13.5 | 2.6 |
Completeness [%] | 99.2 | 98.5 |
Redundancy | 6.8 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 25% PEG3350, 8% Tacsimate |