5GKJ
Structure of EndoMS in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-23 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 128.220, 70.120, 70.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.110 - 3.200 |
| R-factor | 0.23 |
| Rwork | 0.229 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5BSL |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.885 |
| Data reduction software | MOSFLM |
| Data scaling software | MOSFLM |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.920 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.073 | |
| Number of reflections | 10762 | |
| <I/σ(I)> | 17.7 | 10.1 |
| Completeness [%] | 97.6 | 99.9 |
| Redundancy | 6.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.16 M CALCIUM ACETATE, 80 MM SODIUM CACODYLATE, 14.4%(W/V) PEG8000, 20%(W/V) GLYCEROL |
