5GKI

Structure of EndoMS-dsDNA3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-09
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 61 2 2
Unit cell lengths	92.030, 92.030, 405.350
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.720 - 2.900
R-factor	0.186
Rwork	0.182
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5BSL
RMSD bond length	0.009
RMSD bond angle	1.281
Data reduction software	MOSFLM
Data scaling software	MOSFLM
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.720	3.060
High resolution limit [Å]	2.900	2.900
Number of reflections	23761
<I/σ(I)>	16.8	9.2
Completeness [%]	99.8	100
Redundancy	7.2	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.16 M CALCIUM ACETATE, 80 MM SODIUM CACODYLATE, 14.4%(W/V) PEG8000, 20%(W/V) GLYCEROL