5GKE

Structure of EndoMS-dsDNA1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-16
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 61 2 2
Unit cell lengths	92.360, 92.360, 404.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.790 - 2.400
R-factor	0.192
Rwork	0.190
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vld 1dc1
RMSD bond length	0.008
RMSD bond angle	1.233
Data reduction software	MOSFLM
Data scaling software	MOSFLM
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.790	2.530
High resolution limit [Å]	2.400	2.400
Number of reflections	40879
<I/σ(I)>	96.2	9.1
Completeness [%]	98.8	94.6
Redundancy	3.9	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	CALCIUM ACETATE, SODIUM CACODYLATE, PEG8000, GLYCEROL