5GK6
Structure of E.Coli fructose 1,6-bisphosphate aldolase, Citrate bound form
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.033, 73.001, 73.044 |
| Unit cell angles | 90.00, 104.09, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.15907 |
| Rwork | 0.157 |
| R-free | 0.19394 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dos |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.993 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.800 |
| Rmerge | 0.145 | 0.626 |
| Number of reflections | 67229 | |
| <I/σ(I)> | 29.7 | 2.3 |
| Completeness [%] | 98.9 | 99.7 |
| Redundancy | 6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 287 | 0.2M ammonium acetate, 5mM aldose, 0.1M citrate buffer pH 7.0, and 15% PEG 4000 |
