5GJY
Crystal structure of DUCK MHC CLASS I for 1.71 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-05-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 0.97892 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.270, 64.390, 77.920 |
| Unit cell angles | 90.00, 105.74, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.710 |
| R-factor | 0.15878 |
| Rwork | 0.157 |
| R-free | 0.19739 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e0r |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.931 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.710 | 1.800 |
| Rmerge | 0.063 | 0.542 |
| Number of reflections | 41752 | |
| <I/σ(I)> | 20.759 | 2.667 |
| Completeness [%] | 96.2 | 98.2 |
| Redundancy | 4.5 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 8% w/v Polyethylene glycol 1000, 8% w/v Polyethylene glycol 8000, 20% v/v Glycerol |
