5GJU
DEAD-box RNA helicase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 61 |
Unit cell lengths | 57.990, 57.990, 104.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.995 - 1.600 |
R-factor | 0.1687 |
Rwork | 0.167 |
R-free | 0.20360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gxs |
RMSD bond length | 0.010 |
RMSD bond angle | 1.158 |
Data reduction software | MOSFLM |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.221 | 28.995 | 1.690 |
High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
Rmerge | 0.041 | 0.591 | |
Rmeas | 0.091 | 0.045 | 0.645 |
Rpim | 0.036 | 0.018 | 0.255 |
Total number of observations | 161038 | 5012 | 23561 |
Number of reflections | 26166 | ||
<I/σ(I)> | 12.5 | 28.2 | 3.4 |
Completeness [%] | 99.8 | 96.1 | 100 |
Redundancy | 6.2 | 6.1 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.1M DL-Malic acid, pH 7.0, 2mM AMP-PNP |