5GJP

Crystal structure of SrLDC in complex with PLP and Cadaverine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 63 2 2
Unit cell lengths	111.734, 111.734, 112.976
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.2003
Rwork	0.198
R-free	0.27860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gjn
RMSD bond length	0.012
RMSD bond angle	1.636
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.132	0.093	0.317
Number of reflections	14439
<I/σ(I)>	27.8
Completeness [%]	96.8	89.5	95.1
Redundancy	24.2	27.3	12.9
CC(1/2)		0.995	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	293	PEG 200, Sodium phosphate, NaCl