5GJO

Crystal structure of SrLDC mutant (A225C/T302C) in complex with PLP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-13
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	55.917, 122.859, 136.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.1771
Rwork	0.162
R-free	0.19670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gjn
RMSD bond length	0.022
RMSD bond angle	2.055
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.077	0.053	0.298
Number of reflections	87717
<I/σ(I)>	12.7
Completeness [%]	99.3	99	98.3
Redundancy	11.5	12.7	9
CC(1/2)		0.998	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	Ammonium sulfate, Sodium cacodylate, NaCl