5GJK

Crystal Structure of BAF47 and BAF155 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 62 2 2
Unit cell lengths	80.518, 80.518, 200.952
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.475 - 2.052
R-factor	0.1965
Rwork	0.195
R-free	0.21310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aqe 2fq3
RMSD bond length	0.007
RMSD bond angle	0.838
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.050
Number of reflections	25126
<I/σ(I)>	34.9
Completeness [%]	100.0
Redundancy	18.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.4	293	1 M sodium phosphate monobasic monohydrate, potassium phosphate dibasic, pH 5.4