5GIK
Modulation of the affinity of a HIV-1 capsid-directed ankyrin towards its viral target through critical amino acid editing
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.988 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.828, 29.313, 51.291 |
| Unit cell angles | 90.00, 98.58, 90.00 |
Refinement procedure
| Resolution | 53.310 - 1.490 |
| R-factor | 0.18785 |
| Rwork | 0.187 |
| R-free | 0.20963 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.113 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 53.310 |
| High resolution limit [Å] | 1.490 |
| Number of reflections | 19525 |
| <I/σ(I)> | 28.5 |
| Completeness [%] | 75.0 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 1000, reagent alcohol, sodium-phosphate dibasic/citric acid |
