5GI4
DEAD-box RNA helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.617, 86.985, 117.851 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.993 - 2.244 |
| R-factor | 0.2271 |
| Rwork | 0.225 |
| R-free | 0.26830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.102 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.244 | 2.244 |
| Rmerge | 0.074 | 0.607 |
| Number of reflections | 27715 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 98.9 | 95.9 |
| Redundancy | 5.3 | 5.4 |
| CC(1/2) | 0.855 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 2000, 2mM magnesium chloride, 0.1M Tris, 0.2M Trimethylamine N-oxide dihydrate, 2mM AMP-PNP |
