5GI0
Crystal structure of RNA editing factor MORF9/RIP9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979305 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.812, 50.690, 38.318 |
| Unit cell angles | 90.00, 103.53, 90.00 |
Refinement procedure
| Resolution | 29.870 - 2.044 |
| R-factor | 0.2035 |
| Rwork | 0.202 |
| R-free | 0.23520 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.940 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.040 |
| Number of reflections | 9121 |
| <I/σ(I)> | 32.6 |
| Completeness [%] | 99.5 |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 291.15 | sodium bromide,sodium thiocyanate, PEG 3350, MES |
