5GHT

DNA replication protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-17
Detector	RAYONIX MX225HE
Wavelength(s)	1
Spacegroup name	I 2 3
Unit cell lengths	158.133, 158.133, 158.133
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.263 - 2.795
R-factor	0.2054
Rwork	0.203
R-free	0.25070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ghr
RMSD bond length	0.003
RMSD bond angle	0.668
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.795
Number of reflections	16452
<I/σ(I)>	45
Completeness [%]	99.9
Redundancy	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350